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Fig. 9. Sxl raises mitotic rate. Wild-type (A-C); male (D-F) and female (G-I) discs expressing ectopic Sxl in the dorsal compartment by ap-GAL4 driver stained for BrdU incorporation (red) and Ci (green). (E-H) Expansion of Ci in the dorsal compartment identifies discs expressing ectopic Sxl (arrowhead). (D,G) The density of BrdU positive nuclei increases in the dorsal compartment in both males and females (arrow). Scale bars: in C, 20 µm for A-C; in F, 20 µm for D-F; in I, 40 µm for G-I. Wild type (J-L); male (M-O) and female (P-R) discs expressing ectopic Sxl in the dorsal compartment by ap-GAL4 driver stained for PH3 (green) and ß-gal (red; reports dpp expression through dpp-lacZ). Arrowheads in J-R indicate dorsoventral boundary at AP boundary. (M,P) A larger number of PH3 positive cells are found below the dorsoventral boundary relative to J. (N,Q) dpp expression changes in the dorsal half of the disc, which is a little larger than the ventral. Scale bars: in L, 20 µm for J-L; in O, 40 µm for M-O; in R, 40 µm for P-R. Anterior is towards the left, ventral is towards the top. Confocal images throughout.

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