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Fig. 3. Detailed visualization of heart cell migration. (A-H) Transgenic Mesp-GFP embryos. (A) Early tailbud embryo, ~12 hours post-fertilization (hpf), ventral view. (B) Tailbud embryo (~14 hpf), lateral view. (C) Ventrolateral view of a slightly more advanced embryo (~15 hpf). Note that caudal siblings are elongated with punctate accumulations of GFP. (D) Late tailbud embryo (~17 hpf), including magnified views of the two lineages. (E-G) High magnification ventral views of three sets of TVCs from progressively older embryos (~17-18 hpf). (H) Ventrolateral view of an embryo at ~18 hpf. All confocal images display GFP fluorescence in green and are shown with the anterior to the left. In A,C and H, the red channel displays phalloidin staining (Alexa-fluor 647, Molecular Probes). In B and D, the red channel displays auto-fluorescence.





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