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Fig. 3. Detailed visualization of heart cell migration. (A-H) Transgenic
Mesp-GFP embryos. (A) Early tailbud embryo,
12 hours
post-fertilization (hpf), ventral view. (B) Tailbud embryo (
14 hpf),
lateral view. (C) Ventrolateral view of a slightly more advanced embryo
(
15 hpf). Note that caudal siblings are elongated with punctate
accumulations of GFP. (D) Late tailbud embryo (
17 hpf), including
magnified views of the two lineages. (E-G) High magnification ventral views of
three sets of TVCs from progressively older embryos (
17-18 hpf). (H)
Ventrolateral view of an embryo at
18 hpf. All confocal images display
GFP fluorescence in green and are shown with the anterior to the left. In A,C
and H, the red channel displays phalloidin staining (Alexa-fluor 647,
Molecular Probes). In B and D, the red channel displays auto-fluorescence.