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Fig. 4. Activator Mesp fusion protein blocks heart cell migration. (A-C) Transgenic Mesp-GFP embryos. (D-F) Embryos co-electroporated with Mesp-VP16. (A,D) Tailbud embryos ( $~$ 14 hpf) displayed laterally. (B,E) Late tailbud embryos ( $~$ 18 hpf) displayed laterally. (C,F) Late tailbud embryos ( $~$ 18 hpf) displayed ventrolaterally. Embryos are oriented with anterior to the left; GFP fluorescence is in green, phalloidin staining in red. (G,H) Still shots from the movies of transgenic Mesp-VP16 juveniles (Movies 1 and 2 in the supplementary material, respectively). Red arrowhead marks ectopic beating heart tissue; arrow marks the site of tail resorption. The site of typical heart formation is marked by a black arrowhead. E, endostyle; S, stomach. Lateral views with anterior to the left.

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