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Fig. 2. Rho1 localization during oogenesis. Egg chambers. Rho1 (red). DE-Cad (green). (A) Cross-section, stage 2-3. Rho1 accumulates laterally in follicle cells (white arrowhead) and stalk cells (red arrowhead). (B) Stage 10. Rho1 at germ cell boundaries (arrowhead). (C) DE-Cad at apical AJs of follicle cells (blue arrowhead) and in polar follicle cells (white arrow). Rho1 does not preferentially accumulate in either place. (D) Cross-section, stage 10B. DE-Cad and Rho1 elevated apically in centripetal follicle cells (red arrowhead). Rho1 accumulates basolaterally (blue arrowhead). (E) Grazing section basal to AJs, stage 10B follicle cells. DE-Cad is absent from tricellular junctions, where Rho1 accumulates (arrows). (F-I'') Cross-sections, stage 5 (F,G) and 10B (H,I). Wild type (F,H). p120 mutants (G,I). In both Rho1 is enriched along lateral membranes. DE-Cad is enriched at apical AJs (arrowheads). (J,K) Grazing sections, stage 10B. Rho1 at tricellular junctions. Wild-type (J). p120 (K). (L,M) Migrating border cells. Rho1 enriched at plasma membrane and in intracellular vesicles (M, arrowhead). Scale bars: 20 µm in A-F,I,K,L; 5 µm in M.





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