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Fig. 3. ß-Gal expression from hedgehog enhancer constructs. Third
instar eye-antennal imaginal discs, stained for ß-gal antigen, and shown
anterior towards the right and dorsal upwards. Arrowheads mark the
morphogenetic furrow. Transgenic lacZ lines are named as shown in
Fig. 1A and indicated below the
panels, see text. Constructs containing the three adjacent Pointed-binding
sites have robust ß-gal expression posterior to the morphogenetic furrow
(white arrows in A,C,F) but not in the antennal disc or the ocellar region
(black arrows in A,C,F). The 203 bp fragment bar3L2 is sufficient to
drive expression of lacZ reporter in the eye (F). Mutation for all
three Pointed-binding sites eliminates this reporter expression (G). Six
tandem Pointed-binding sites are not sufficient to drive expression of
ß-gal (H). Scale bar: 50 µm.
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