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Fig. 5. pointed regulates the expression of hedgehog from an eye specific enhancer. (A-D) Third instar bar3:lacZ eye imaginal disc. The field shown is several columns posterior to the morphogenetic furrow, anterior towards the right. The disc contains a mosaic clone that is homozygous for the null mutation pntdelta88. (A,D) GFP negatively marks the outlined clone. (B,D) Elav marks all the photoreceptors. (C,D) lacZ reporter. lacZ reporter expression is absent from within the pointed clone (a wild-type example outside the edge of the clone is indicated with a white arrow). The single R8 cells are Elav positive in the clone (black arrow). Scale bar: 5 µm.





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