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Fig. 6. Pointed P2 protein binds specifically to the hedgehog eye specific enhancer. Electrophoretic mobility shift assay (EMSA) gel for GST-Pointed binding. Competitor DNA is indicated at the top, with the rising level indicated by ramps. The presence or absence of GST-Pointed is indicated by + or –. Lane numbers are indicated. Lane 1, probe alone; lane 2, no competitor; lanes 3-7, 203 bp Bar3L2 competitor, ranging from 15 to 240 times molar excess; lanes 8-12, 203 bp Bar3L2(deltaETS) competitor, ranging from 15 to 240 times molar excess; lane 13, cold probe as competitor and lane 14 Ets site mutated cold probe as competitor. The probe sequence is shown below gel with the three Ets consensus sites highlighted. The nine mutations made in Bar3L2(deltaETS) are shown in white boxes.





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