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Fig. 1. Isolation and dominant-interfering activity of a truncated Zic2
cDNA. (A) Upper panel: diagram showing the Xenopus Zic2 protein
compared with the tZic2 protein. Lower panel: partial alignment of the amino
acid sequences of the full-length and truncated-Zic2 proteins. Two potential
starting methionines are boxed. The start of the zinc-finger domain is
underlined. (B) In vitro translation of tZic2. Two preparations of tZic2
plasmid were translated in transcription-translation coupled reactions.
Luciferase (Luc), 
50 kDa. (C) Injection of tZic2 causes
microcephaly and cyclopia. Uninjected and tZic2-injected (500 pg)
embryos at stage 40. (D) Hematoxylin and Eosin stained sections from the
embryos in C showing reduced forebrains (large arrowhead) and abnormal or
cyclopic eyes (small arrowhead). (E) Real-time RT-PCR analysis of neural crest
(Slug, Sox9, Twist) and neural (NCAM) marker expression in
animal caps cultured to stage 22. Samples and amounts injected are indicated
in the key. Relative expression values; samples were normalized to
ODC and expressed as a percentage of a whole stage 22 embryo
sample.
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