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Fig. 6. Rescue of Zic2-depletion by injection of CerS. (A) Analysis
of microcephaly (microceph.) and axial truncations (axial trunc.) in control
stage 33 embryos (Un), controls injected with 50 pg CerS mRNA
(Un+CerS), Zic2-depleted embryos (Zic2-) or
Zic2-embryos injected with 50 pg CerS mRNA (Zic2-,
+CerS). Data were pooled from two host-transfer experiments. Bars
represent the number of embryos with the indicated phenotype. Axial
truncations were present in a subset of embryos with microcephaly. (B)
Expression of Xnr1, Xnr5 and Xnr6 in gastrula (stage 10.5)
stage embryos analyzed by RT-PCR. (C,C') Examples of embryos from the
experiment in B. Zic2-embryos show both microcephaly and axial
truncation; the Zic2-, +CerS embryos represent a normal and
a microcephalic embryo (upper and lower embryo, respectively).
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