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Fig. 2. Tamoxifen-induced deletion of the Hoxa2 locus. (A-F) In-situ
hybridization on whole mounts (A,B) and frontal cryosections (C-F) of mouse
embryos using an antisense Hoxa2 probe. Wild-type embryos at 10.5
(A), 13.5 (C) and 14.5 (E) dpc show strong Hoxa2 expression in the
second (BA2) and posterior branchial arches (A), as well as in the neural tube
(NT) and middle ear regions (C,E), respectively. Significant loss of
Hoxa2 transcripts is observed in 10.5 dpc
Hoxa2flox/flox;Cre-ERT2 embryos (i.e. carrying two alleles
to be excised) after tamoxifen (TM) treatment at 9.5 dpc (B), as well as in
13.5 (D) and 14.5 (F) dpc Hoxa2del/flox;Cre-ERT2 embryos
(i.e. carrying only one allele to be excised) after TM treatment at 11.5 and
12.5 dpc, respectively. (G,H) Immunostaining on frontal cryosections of
Hoxa2EGFPfloxNeo;CMV-Cre (G) and
Hoxa2EGFPfloxNeo;Cre-ERT2 (H) 13.5 dpc mouse embryos using
an anti-GFP antibody. Note that the distribution of TM-induced GFP expression
in H is comparatively similar to the constitutive GFP expression in G. OC,
otic capsule; S, stapes.
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