|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Correct retinal NT patterning after heterotopic optic vesicle
transplantation. (A-C) Transplantation scheme. At the 5- to 7-somite stage,
optic vesicles were transplanted from the forebrain of donor embryos onto the
yolk cell of hosts (B) by slipping the graft in between the locally-opened
epidermis and the yolk cell membrane (C). A live image of the anterior neural
keel (dorsal view) in shown in A, (D,E) Live host embryos: heterotopic grafts
develop and differentiate on the yolk cell and form eyes of normal morphology.
(F-I) Retinal NT patterning after heterotopic transplantation at 28 hours of
development: in grafts, the nasal expression domain of efna5a (F) and
the temporal expression domain of epha4b (H) are established and
maintained normally in the ectopic situs (G,I) of the embryo. (F,H)
Flat-mounts of dissected eyes, orientation as indicated in F. Arrowheads
indicate the implantation site (D,E,G,I) and the ventralmost position of the
eye (F,H). Broken outline in A indicates grafted tissue; in F,H it indicates
the lens. A, anterior; P, posterior; g, graft; hpt, hours
post-transplantation; D, dorsal; V, ventral; N, nasal; T, temporal; Pr,
proximal; Di, distal.
|
