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Fig. 5. Drosophila Tral and Me31B form a RNA-dependent complex and
colocalize in the germline. (A) RNA-dependent interaction between
Drosophila Tral (CAR-1 ortholog) and Me31B (CGH-1 ortholog). Extracts
from Drosophila ovaries were immunoprecipitated with a Me31B antibody
(Nakamura et al., 2004), and
analyzed for the presence of Tral by western blotting. Co-immunoprecipitation
of Tral with Me31B (lane 3) was abolished by RNase A treatment (lane 4). Lane
2 shows protein extract incubated with control IgG. (B-D) Extensive
colocalization (D) of Me31B (B) and Tral (C) in cytoplasmic granules in the
Drosophila germline. A stage-7 egg chamber expressing GFP-Me31B was
stained with a Tral-specific antibody.
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