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Fig. 7. The effect of Exp mutations on the defecation cycle length in wild-type and
clk-1 mutant backgrounds. The bars represent the mean of the mean
cycle lengths of n animals that had each been scored for five
consecutive defecation cycles; the error bars represent the standard deviation
of the animal means (n>10 animals). (A) In a wild-type (N2)
background, both dsc-1(qm133) and exp-1(sa6) significantly
shorten the defecation cycle; unc-25(e156) and hlh-8(nr2061)
significantly lengthen the cycle. All the Exp mutants show an oscillation in
cycle length, with the cycles getting progressively shorter as the worm
becomes increasingly constipated (Con), which eventually produces a forceful
expulsion, and is then followed by one or two longer cycles. This phenotype is
revealed by the variability of the cycle length in individual animals scored
for five cycles. This can be expressed as a per animal standard deviation. The
average per animal standard deviations are as follows: N2: 0.89,
dsc-1: 1.90, exp-1: 3.43, unc-25: 3.92 and
hlh-8: 11.68. (B) Of the four Exp mutants surveyed, only the
dsc-1(qm133) mutation can significantly suppress the lengthened
defecation cycle of clk-1(qm30) mutants. The asterisks indicate the
data that are significantly different from N2 (A) or clk-1 (B). All
significant differences detected were at a level of P<0.0001.
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