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Fig. 2. Overexpression of ey or toy is not capable of altering PE
fate. Anterior is towards the left in all panels, and dorsal is upwards
(except for z-sections in B and D). (A,B) tsh-expressing PE
clones (arrows) upregulate Ey expression (A) and Eya (B). In some
tsh-expressing clones, the upregulation of ey is not
strictly cell-autonomous, as some cells adjacent to the expressing clone also
increase Ey signal (A, arrowhead). (B) Confocal z-section through the
white line is shown, demonstrating the PE location of the tsh+ clone (arrow),
the cell-autonomous activation of Eya (green) and the repression of Hth
(blue). Normal Eya expression is seen in the ME (me). (C) An
ey-expressing clone in the peripodial epithelium (red), though, does
not activate eya expression (green) or alter the morphology of the
peripodial cells. (D,E) toy-expressing PE clones (toy+; arrowheads),
marked with lacZ (blue). (D) toy-expressing PE cells (blue)
upregulate Ey expression, while that of Eya remains absent. Separate channels
of a z-section through the clone marked with the arrowhead are shown
on the right. Ey and Eya are detected normally in the ME (me). The white line
indicates the approximate location of the section. (E) In PE
toy-expressing clones (arrowheads), Tsh is not upregulated.
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