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Fig. 3. tsh induces competence to respond to dpp and wg
in peripodial cells. (A-C,F-H,J) PE clones. Anterior is towards the left, and
dorsal upwards. (A) Clones expressing an activated tkv receptor
(tkvQD+, red) do not activate Eya expression (green) or induce
cell-morphological changes (arrows); in addition, Hth expression remains
unchanged (blue). (B) Posterior tsh-expressing peripodial clones
(Tsh+) induce Eya expression (arrows), but anterior ones do not (arrowheads).
(C) In tsh-expressing clones simultaneously mutant for the
dpp signal transducer Mad (Tsh+ Mad-; arrows) eya
is never induced. (D,E) ME clones (arrows): (D) tkvQD-expressing
clone (red) in anterior regions of the disc de-represses Eya (green; the
coexpression is seen in yellow). These clones lose Hth expression (blue). (E)
Conversely, a Mad– clone (marked by the absence of
lacZ, in red) shows a strong reduction of Eya signal. (F)
Axin-expressing clones (lacZ, red; arrows) grow normally in
the PE, and do not affect Hth expression (green; overlap in yellow). (G) A
tsh-expressing PE clone (tsh+; marked with Tsh in red;
arrow) shows overgrowth, with more compact nuclei that strongly express Hth
(overlap in yellow). (H) Cells in a clone coexpressing Axin and
tsh (tsh+ Axin+, marked with Tsh in red; arrow) lose Hth expression
and do not overproliferate. (I) An anterior ME Axin-expressing clone
(lacZ in red; arrow) lying within the tsh domain (blue)
downregulates Hth (green). Hth levels decrease towards the posterior of the
clone, as Axin+ cells are farther away from the anterior wg
expression domain. In these Axin+ clones, Tsh expression is maintained
(co-expression seen in magenta). (J) PE clones expressing simultaneously
tsh, Axin and tkvQD (marked with Tsh, red) activate Eya
expression both in posterior (arrow) and in anterior (arrowheads)
locations.
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