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Fig. 3. Nectaries of the Brassicales. Nectaries and expression of CRC orthologs in
Lepidium africanum (A-D), Cleome sparsifolia (E-G) and
Capparis flexuosa (H-P). (A) Floral nectaries (n) of Lepidium
africanum are located between stamens and petals (p). Nectary glands
flank each of the two stamens, such that each flower has four glands. (B)
Nectary glands have apical stomata (arrow). (C,D) LaCRC is expressed
in all cells of the nectary glands (arrow in C), from inception beyond
anthesis. (E,F) Cleome sparsifolia floral nectaries (arrow) form a
ring at the abaxial bases of the stamens. (G) CsCRC is expressed at
high levels throughout nectaries. (H,I) Extrafloral nectaries of Capparis
flexuosa (arrows) are located in leaf axils and are covered with scales,
dissected away in I. (J) Longitudinal section of an extrafloral nectary
reveals the internal anatomy (arrow). An axillary bud is adjacent and below
the extrafloral nectary. (K) The covering sheath is composed mostly of
thickened collenchyma cells and fibers, and the nectary has stomatal openings
on its surface (arrows). (L) Nectaries comprise mostly parenchyma cells with
phloem traces containing sieve tube members and companion cells. (M-P) Before
nectary tissue develops inside the sheath, CfCRC expression is not
observed (M, the dark spot is not signal). As nectary tissue initiates
development, CfCRC expression increases dramatically and levels
remain high throughout the nectary (N,O). As nectary growth ceases
CfCRC expression decreases and only a minimal expression level
remains (P).
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