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Fig. 6. Overexpression of rnt-1 drives extra seam cell divisions. (A)
Control wild-type worms subjected to heat shock (33°C, 1 hour) have the
normal number of seam cells, visualised in adults with the scm:GFP
reporter (white arrow). Scale bar: 70 µm. (B) Adult hermaphrodite carrying
the hsp16-2::rnt-1 construct, previously subjected to heat shock
(33°C, 1 hour) in L3. Several extra seam cells are visible with the
scm::GFP reporter (white arrow). Anterior is towards the left in both
panels. Scale bar: 70 µm. (C) Graph showing a quantitative analysis of
adult seam cell number in heat shocked worms. Wild-type hermaphrodites
subjected to heat shock (33°C, 1 hour) during L2 or L3 have the normal
adult number of seam cells (data not shown). Transgenic worms carrying the
hsp16-2::rnt-1 construct display the normal adult seam cell number
following no heat shock (n=30), or heat shock during L1
(n=42) or L4 (n=25), but have extra seam cells following
heat shock during L2 (n=31, P<0.01) or L3 (n=48,
P<0.00001). Error bars represent the standard error of the mean.
(D) him-5(e1490) male carrying the hsp16-2::rnt-1 construct.
This animal had been previously subjected to heat shock during L2. An extra
ray 3 is visible on one side of the animal, fused to the expected single ray 3
(white arrows). Scale bar: 10 µm
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