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Fig. 6. Rib cartilage differentiation in wild-type mice. Orientation and location
of the sections in this figure are indicated in the bottom left-hand panel of
the figure (black rectangle represents transverse section, red arrow
represents longitudinal section). (A,B) Transverse sections through the rib
cartilage stained with Safranin O. (A) Section through rib of 1-week-old
mouse. Chondrocytes have a uniform undifferentiated appearance. (B) Beyond 2
weeks of age, chondrocytes in the center of the element become hypertrophic,
while cells in the periphery remain undifferentiated. (C-G) In situ
hybridization with probes for Col2 and Col10. (C,D) Until 1 week of age, all
chondrocytes, except for the hypertrophic chondrocytes of the growth plate,
express Col2. (E-G) At 2 weeks of age, chondrocytes in the center of the rib
cartilage turn off expression of Col2 (area indicated by yellow arrows in E
and F), while cells in the periphery, near the perichondrium, continue to
express Col2. Chondrocytes in the center now express Col10 (G). GP, growth
plate. Scale bars: 100 µm.
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