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Fig. 7. (A) Safranin O stained longitudinal section of a rib of a 2-week-old
wild-type mouse is shown. Rectangle indicates area of interest representative
for B and C. (B) Rib of a Ext2+/–. Arrow indicates
the location of a chondrocyte nodule. Asterisk indicates location of the
proliferative zone of the rib growth plate. The inset in the top right-hand
corner shows a magnification of the nodule. (C) BrdU staining of adjacent
serial section shown in B. BrdU-positive cells can be seen in the
proliferative zone of the growth plate of the ribs (arrowhead). The
chondrocyte nodule does not contain BrdU-positive cells (arrow). (D,E) Higher
magnification of the area with the nodules shown in B and C. (D) In situ
hybridization with Col10 antisense probe shows expression by chondrocytes
located in the center of the rib cartilage. The nodule of displaced
chondrocytes (encircled by the broken line) expresses Col10 and are separated
from the centrally located hypertrophic cells by a layer of chondrocytes that
do not express Col10. (E) Staining with the HS antibody 10E4 shows that the
chondrocytes in the nodules (encircled by the broken line) produce HS. Inset
in E shows staining with 10E4 after heparinase treatment. Scale bars: 200
µm in A-C; 50 µm in D,E.
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