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Fig. 9. Examination of the Ext2+/– long bone growth
plate. Growth plates from ulnae of E18.5 wild type and
Ext2+/– were analyzed for proliferation and
differentiation defects. (A,B) Safranin O staining shows a similar overall
structure of the growth plates of a wild-type and
Ext2+/– mice. (C,D) Magnification of the rectangular
areas in A and B, respectively. Close examination of the chondrocytes in the
proliferative zone shows a loss of the characteristic columnar organization
normally seen in wild-type growth plates (arrow indicates typical columnar
organization). (E,F) Von Kossa stained sections shows the presence of
mineralized tissue in the growth plates of both genotypes. (G,H) BrdU labeling
shows a similar number of labeled cells in the proliferating zone of the
growth plates of wild-type and Ext2+/– mice. (I-P)
In situ hybridization with markers for chondrocyte differentiation show
Ext2+/– mice expressed Ptch1, Ihh, Pthr1 (PPR) and
ColX in appropriate places but there is a small decrease in the expression
domain of Pthr1 and Ihh (compare arrow in K with arrow in L, and arrow in M
with arrow in N). P, Proliferating chondrocytes; Ph, Pre-hypertrophic
chondrocytes; H, Hypertrophic chondrocytes. Scale bars: 100 µm in A,B,E-P;
50 µm in C,D.
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