DEV02075 Supplementary Material

Files in this Data Supplement:

• Supplemental Table - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Confocal images of cryosectioned retinae from ptf1a::GFP transgenic zebrafish immunostained with zn5 antibodies to label ganglion cells (red). Phallodin labeling of cell membranes (blue). (A) At 36 hpf, zn5-labeled ganglion cells are located at the inner edge of the retina. GFP⁺ presumed amacrine cells can be seen at various depths of the retinal neuroepithelium, but not at the inner edge of the retina. (B) At 48 hpf, the population of GFP⁺ amacrine cells lies at the interface of the population of zn5⁺ ganglion cells. A plexus (blue) can be seen emerging between the two populations of GFP⁺ amacrine cells: elongated sclerally located and rounded vitreally located amacrine cells.

• Supplemental Figure 2 -

  Fig. S2. A second example of a displaced amacrine cell in a ptf1a::GFP retina with neurites oriented towards the INL from early time-points. Time-lapse in vivo confocal images of a vitreally positioned GFP⁺ amacrine cell (green) co-labeled red by an a-tubulin promoter driving expression of mCherry.

• Movie 1 -

  Movie 1. In vivo time-lapse recording of GFP⁺ amacrine cells from ptf1a::GFP transgenic fish. While the overwhelming majority of GFP⁺ neurites is concentrated between the two populations of amacrine cells, a small number of neurites can be seen extending towards the GCL. Each image is a maximum projection of six optical planes encompassing a depth of 2 µm. Images were acquired every 30 minutes between 41 hpf and 52 hpf.

• Movie 2 -

  Movie 2. In vivo time-lapse recording of a pair of CFP⁺ amacrine cells, depicted in Fig. 4A, from line Q11. Neurite outgrowth occurs from all parts of the somata, directed towards the GCL, towards the outer retina and laterally. Each image is a maximum projection of optical planes (0.7 µm) encompassing the cells. Images were acquired every hour between 56 hpf and 64 hpf.

• Movie 3 -

  Movie 3. In vivo time-lapse recording of a CFP⁺ amacrine cell, depicted in Fig. 4B, from line Q11. For each time-point, neurites were scored as directed towards the GCL, the OLM or sideways (S), and plotted in Fig. 4C. Each image is a maximum projection of optical planes (0.7 µm) encompassing the entire cell. Time-lapse movie of six frames show images used for neurite analyses. These images were acquired at 55 hpf (0¢), and 30, 60, 135 and 195 minutes later.

• Movie 4 -

  Movie 4. Within the IPL, extensive remodeling of amacrine cell arbors occurs. In vivo time-lapse recording of a YFP⁺ amacrine cell from line Q14 showing growth and retraction in different portions of its arbor. Each image is a maximum projection of optical planes (0.7 µm) encompassing the cell. Images were acquired every 5 minutes beginning at 56 hpf.