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Fig. 4. Exuberant neurite outgrowth continues even when amacrine cells are located near or at their final somal positions. (A) Time-lapse confocal images of a pair of CFP+ amacrine cells from line Q11 reveal neurites directed appropriately towards the GCL, and erroneously towards the outer retina. Imaging commenced at 56 hpf (0') and proceeded to 64 hpf (480'). To examine whether the neurite extension of amacrine cells located near their final somal positions displayed directionality, we scored neurites as being directed towards the GCL, the OLM or sideways (S), as shown for the amacrine cell in B. For the cell in B, during the period of recording, the number of neurite tips directed towards the GCL was consistently found to exceed the number of neurite tips directed elsewhere (C). (D) Histograms of a similar analysis for six cells (each differently shaded), where the number of neurites was averaged over a period of 90 minutes.





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