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Fig. 7. Amacrine cells that ultimately stratify in the OFF sublamina display an early bias for the outer half of the IPL. (A) Confocal time-lapse images of a YFP⁺ amacrine cell from line Q08 (green) in the background of the ubiquitously-expressing CFP line Q01 (red). The amacrine cell confines its lateral arbors to the outer part of the IPL from the earliest time points (57 hpf) until the time when it is stratified (80 hpf). (B) Early restriction of neurites to the outer half of the IPL for OFF amacrine cells can be better appreciated for a cell (GFP line 244, green) that arrives at the interface of the IPL (right cell in all panels) demarcated by the ubiquitous expression of CFP (line Q01, red). (C) Morphometric analysis of 10 GFP⁺ or YFP⁺ cells imaged in the background of line Q01 confirmed that the vast majority of GFP⁺ or YFP⁺ pixels lies in the outer half of the IPL early (57-62 hpf) and late (70-72 hpf), for presumed OFF amacrine cells.

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