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Fig. 1. CYC protein binds to DNA. (A) Specificity of CYC recombinant protein
binding to class I and class II TCP consensus binging sites. Oligonucleotide
probes contain a shared consensus binding site for class I GGNCCCAC (red) and
class II GTGGNCCC (blue) to which CYC protein binds (arrow; lane 1). Bold
underlined nucleotides indicate mutations introduced that affect both class I
and II binding (lanes 2 and 3) or specifically only class II (lane 4) or class
I (lane 5). (B) Consensus DNA-binding sequence for CYC protein obtained by a
random binding site selection method. After five rounds of selection, a total
of 31 binding sites of CYC were piled up using MEME
(meme.sdsc.edu)
and a consensus sequence generated. Nucleotides that were not occupied by a
specific residue are indicated by N.
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