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Fig. 2. Interaction of CYC recombinant protein with RAD promoter and
intron. (A) RAD genomic region showing three potential CYC-binding
sites in the promoter and intron. (B) PCR fragments of promoter and intron
sequences with putative CYC-binding sites were amplified and used as probes in
an EMSA with recombinant CYC protein. + or indicates the presence or
absence of CYC protein; asterisks indicate retardation of the mobility of
CYC-DNA binding complex.