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Fig. 3. Shh inactivation in the peripheral retina is associated with
increased RGC development. (A-D) Retinas from wild-type and
-Cre;Shh–/c mice at E17 stained for BrdU and
Brn3b. RPC were labelled in vivo with BrdU at E16 and harvested 24 hours
later. B and D are high magnification views of the neuroblast layers in A and
B, respectively. Arrows in D indicate examples of double-labelled cells. The
layer of Brn3b+ RGCs is thickened and there is an increase in
double-labelled cells in the -Cre;Shh–/c
retina (C,D). (E) Quantification of the number of Brn3b+ cells in a
300 µm region in the central and peripheral regions of wild-type and mutant
retinas at P0. (F,G) Birthdating analysis in the embryonic
-Cre;Shh–/c retina. RPC were labelled in vivo
with BrdU at E13 and the retinas were harvested at P0. The number of
Brn3b+ cells with intense BrdU-labelling (F) and the proportion of
Brn3b+ cells among the heavily BrdU-labelled cohort (G) were
quantified in 300 µm regions of the central and peripheral retinas in
wild-type and mutant mice. *P<0.005. Scale bar: 100 µm in A,C;
50 µm in B,D.
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