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Fig. 5. Shh inactivation affects differentiation and the development of
late-born cell types. Retinal sections from wild-type (A,C,G,I,K,M,O) and
-Cre;Shh–/c mice (B,D,H,J,L,N,P) were stained
at P0 for syntaxin (A,B) to identify amacrine and horizontal cells; at P6 for
cone-arrestin (C,D) to identify cone photoreceptors; at P9 for Hoechst (G,H)
to mark nuclei and for PKC (I,J) to identify rod bipolar cells; and at P6 for
Hoechst (K,L), rhodopsin (M,N) to identify rod photoreceptor cells and CRALBP
(O,P) to identify Müller glia. Red arrows in B indicate the line of
differentiating horizontal cells in the outer plexiform layer in the periphery
of the -Cre;Shh–/c retina. Horizontal cells
are not differentiated in the control retina at this stage (A). The intensity
of cone arrestin staining is greater in the periphery of the
-Cre;Shh–/c retina compared with wild-type
littermates (C,D). (E) Quantification of retinal cell types in the central and
peripheral regions of the wild-type and mutant retinas at P1 (rods), P6
(cones, Müller glia) and P9 (bipolar cells). (F) The distribution of
cells between the different retinal layers in a 100 µm region of the
central and peripheral retinas of wild-type and mutant mice at P6. (G-J)
Bipolar cells are reduced in number and disorganized in degenerating periperal
retinas of -Cre;Shh–/c mice.
**P<0.05, *P<0.005. (K-P) Degenerative changes take
place in the periphery of the -Cre;Shh–/c
retina, as indicated by the rosettes (arrows in L), gaps in rhodopsin staining
(arrows in N) and infiltration of Müller glial cell bodies into the ONL
(arrows in P). Scale bar: 100 µm.
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