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Fig. 6. Ectopic Hh pathway activation restores cell cycle gene expression and bipolar cell development in retinal explants from {alpha}-Cre;Shh–/c mice. Retinal explants from wild-type and {alpha}-Cre;Shh–/c mice at P1 were cultured for 2 (A-L) or 9 (M-X) days and processed for in situ hybridization to detect mRNA for Gli (A-D), cyclin D1 (E-H) and Chx10 (I-L) or stained for Hoechst to reveal nuclei and anti-PKC (M-P), anti-CRALBP (Q-T) and anti-syntaxin (U-X) antibodies. The peripheral region of the explants is shown in these panels. Scale bar: 100 µm.





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