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Fig. 7. Shh signalling modulates Hes gene expression in RPC. (A,B) In situ hybridization for Hes1 mRNA in the retina of wild-type (A) and {alpha}-Cre;Shh–/c (B) mice at P0. Hes1 expression is reduced in the peripheral retina of the {alpha}-Cre;Shh–/c (between the red arrows). (C) Semi-quantitative RT-PCR analysis of gene expression in control E18 retinas and in E18 retinal explants cultured for 1 and 3 days under the indicated conditions. Scale bar: 100 µm in A,B.





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