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Fig. 3. Expression of EphB2, EphB3, ephrin B2 and ephrin B1 in uninfected and RCAS-DNhRAR ${alpha}$ infected retinae. In situ hybridization carried out on flat-mounted E7 retina either uninjected or injected with RCAS-DNhRAR ${alpha}$ . Retinae shown with dorsal towards the top, ventral towards the bottom, anterior (nasal) towards the left and posterior (temporal) towards the right. In situ hybridization of uninjected E7 retina, with EphB2 (A), EphB3 (E), ephrin B2 (I) and ephrin B1 (M). Double in situ hybridization of RCAS-DNhRAR ${alpha}$ injected E7 retina with EphB2 and RCAS probes (B-D). Magnified view of the boxed area from B showing EphB2 expression (purple signal, C) and RCAS infection (blue signal, D). RCAS-DNhRAR ${alpha}$ injected E7 retina with double in situ hybridization for EphB3 and RCAS probes (F-H). Magnified view of the boxed area in F showing EphB3 expression (purple signal, G) and RCAS-infected area (blue signal, H). RCAS-DNhRAR ${alpha}$ -injected E7 retina with double in situ hybridization for ephrin B2 and RCAS probes (J-L). Magnified view of boxed area in J showing ephrin B2 expression (purple signal, K) and RCAS infected area (blue signal) (L). RCAS-DNhRAR ${alpha}$ injected E7 retina with double in situ hybridization for ephrin B1 (purple signal, N) and RCAS (blue signal, O). The white arrows indicate infected areas with subtle downregulation of ephrin B1 expression.

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