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Fig. 2. Nkx2.1+/Th+ hypothalamic DA neurons are generated from Nkx2.1+/Shh+
progenitors in the lateral tuberal hypothalamus. (A,B) Transverse sections
through embryonic day 6 (E6) tuberal hypothalamus (shown in A'),
immunolabelled with Th (green) (A), Shh (red) (B) or both (B'). Th and
Shh show extensive overlap. Blue shows DAPI counterstain. (C-F) Fate mapping
of germinal zone of lateral tuberal hypothalamus. (C) Schematic, showing DiI
labelling at stage 15. (D-F) Transverse sections through DiI-labelled embryos,
fixed immediately (D) or developed to E6 (E,F). Immunolabelling with anti-Shh
confirms that lateral tuberal cells are targeted at stage 15 (arrow, D) and
remain in situ at E6 (arrow, E). Immunolabelling with anti-Th shows that
DiI-labelled cells (red) co-label with anti-Th antibody (green; arrows in F).
(G-I) Transverse sections through E6 tuberal hypothalamus, immunolabelled with
Th (green) and Nkx2.1 (red) (G,H) or Th (red) and Lim1 (green) (I). Many Th+
neurons co-express Nkx2.1. Likewise, extensive co-expression of Th and Lim1 is
detected. (J-L) Confocal image showing overlapping expression of Nkx2.1 and
Msx1 in the lateral tuberal hypothalamus. Scale bars: 140 µm in A,B,G; 60
µm in D; 120 µm in E; 20 µm in F; 30 µm in H; 25 µm in J-L.
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