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Fig. 3. MSP-binding site distribution and expression of GFP::NMR-1 and VAB-1::GFP
in the proximal gonad. (A) Fluorescein-labeled MSP (MSP-FITC) binds to oocytes
and sheath cells of proximal gonads. In deconvolved mid-focal plane images,
fluorescent intensity is enriched at the oocyte/oocyte interface. (B) A 3D
reconstruction of the stack shows similar results. (C) The fluorescent
intensity is high at membrane sites between oocytes even when the sheath cells
are removed. (D) By contrast, MSP-FITC appears uniformly distributed around
the oocyte plasma membrane in isolated oocytes. (E-G) In GFP::NMR-1-expressing
gonads, GFP fluorescence is observed at the oocyte plasma membrane (E).
Extensive co-localization with F-actin (F) is observed in the merged image
(G). (H-J) VAB-1::GFP expressing gonads show a similar pattern of
fluorescence. (K-M) GFP fluorescence is not observed in control gonads. (N,O)
By focusing on the sheath membrane, GFP fluorescence is detected in the sheath
of GFP::NMR-1-expressing gonads (N), but not in control gonads (O). Arrows
indicate the sheath cells. All gonads are oriented as shown in
Fig. 1A. DAPI is included in
E-M. The VAB-1::GFP expression pattern has been reported previously
(Miller et al., 2003). Scale
bars: 10 µm.
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