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Fig. 3. Molecular characterization of Ihh^–/–;Gli3^–/– limbs. Serial sections of E16.5 wild-type (A,D,G,J), Ihh^–/– (B,E,H,K) and Ihh^–/–;Gli3^–/– limbs (C,F,I,L) were hybridized with riboprobes as indicated. (B) In Ihh^–/–;Gli3^–/–-deficient mice Col10a1 expression is restricted to the center of the skeletal elements, indicating a delayed onset of hypertrophic chondrocyte differentiation compared to Ihh^–/– mice. (E,H,K) The Ihh target genes Ptch, Gli1 and PTHrP are not expressed in Ihh^–/– mice. (F,I,L) In Ihh^–/–;Gli3^–/– limbs the expression of Ptch and PTHrP, but not Gli1, is partially rescued. (F) Ptch is weakly expressed in proliferating chondrocytes. (J,L) PTHrP expression can be detected in the perichondrium (red arrow) and in the carpal joints (green arrowhead). No PTHrP expression can be detected in the distal chondrocytes (yellow arrowhead).

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