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Fig. 5. Gli3 regulates distal chondrocyte differentiation. Two sets of serial
sections, A,B,D,E and G,H,J,K, of E16.5 wild-type (A,D,G,J) and
Gli3–/– (B,E,H,K) limbs were hybridized with
riboprobes as indicated. Red dashed lines indicate the distal border of the
Ihh expression domain. (C,F,I,L) Models of gene expression domains.
(A-C) Gli3–/– mice display a reduced zone of
proliferating chondrocytes compared to wild-type mice (red arrow). (D-F)
PTHrP is expressed at similar levels in
Gli3–/– and wild-type limbs, however its
domain of expression is shifted towards the distal end of the cartilage
anlagen in Gli3–/– limbs. (G-L) In
Gli3–/– limbs the Fgfr1 expression
domain (G-I) is reduced (green arrow) and the Fgfr3 expression domain
(J-L) is shifted towards the end of the skeletal elements (blue arrow),
indicating an accelerated differentiation of distal chondrocytes. (M) The zone
of proliferating chondrocytes (red arrow in A-C) is shortened in
Gli3–/– limbs
(***P=0.00003, n=6). The
PTHrP-expressing zone of distal chondrocytes (yellow arrow in D-F) is
reduced in Gli3–/– limbs
(*P=0.00008, n=6). The distance between the
Ihh expression domain and the central end of the PTHrP
expression domain (gray arrow in D-F) is of similar size in both,
Gli3–/– and wild-type limbs
(**P=0.304, n=6). (N) The zone of
Fgfr1-expressing distal chondrocytes (green arrow in G-I) is reduced
in Gli3–/– mice
(*P=0.0002, n=5), whereas the zone of
Fgfr3 expression (blue arrow in J-L) is of similar size
(**P=0.19, n=5).
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