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Fig. 6. (A-D) In Ihh–/– limbs distal chondrocytes
differentiate directly into prehypertrophic chondrocytes. Sections of E14.5
wild-type (A,C) and Ihh–/– (B,D) limbs were
hybridized with Fgfr1 (A,B) and Pthr1 (C,D) riboprobes. In
Ihh–/– mutants Fgfr1 is expressed in
a stripe of distal chondrocytes, which is flanked by Pthr1 expressing
hypertrophic chondrocytes. Black dashed lines indicate the border between
Fgfr1 and Pthr1 expression domains; red dashed lines
indicate the end of the cartilage elements. (E-H) Loss of Gli3
restores columnar chondrocyte differentiation in
Ihh–/– mice. Sections of E16.5 wild-type (E,G)
and
Gli3–/–;Ihh–/–
(F,H) limbs were hybridized with riboprobes for Fgfr1 (E,F) and
Pthr1 (G,H). In
Gli3–/–;Ihh–/–
mutants a reduced zone of Fgfr1 expressing distal chondrocytes is
separated from the Pthr1 expression domain by a population of
columnar chondrocytes. B,D and F,H display serial sections.
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