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Fig. 8. Loss of Gli3 partially rescues the Col2a1-Ihh phenotype. Serial
sections of E16.5 Gli3+/– (A,E,I,M,Q; control),
Gli3–/– (B,F,J,N,R),
Col2a1-Ihh;Gli3+/– (C,G,K,O,S) and
Col2a1-Ihh;Gli3–/– (D,H,L,P,T) limbs of the
same litter were hybridized with riboprobes as indicated. E-H are higher
magnifications of the distal radii (boxed) in A-D. (E-H) The size of the zone
of proliferating chondrocytes (red arrow) is reduced in
Gli3–/– mice and strongly increased in
Col2a1-Ihh;Gli3+/– mice. Compared to
Col2a1-Ihh;Gli3+/– mice, the zone of proliferating
chondrocytes is decreased in double transgenic
Col2a1-Ihh;Gli3–/– mice. (I-L) The size of the
domain of distal chondrocytes, demarcated by the Fgfr1 expression
domain (green arrow), is slightly reduced in
Col2a1-Ihh;Gli3+/– and strongly reduced in
Gli3–/– and
Col2a1-Ihh;Gli3–/– mice. (M-P) The
Fgfr3 expression domain is expanded in
Col2a1-Ihh;Gli3+/– limbs, whereas in double
transgenic Col2a1-Ihh;Gli3–/– mice the
Fgfr3 expression domain is of similar size to that in wild-type and
Gli3–/– limbs. (Q-T) The upregulated level of
PTHrP expression in Col2a1-Ihh;Gli3+/– mice
seems to be downregulated in
Col2a1-Ihh;Gli3–/– mice to similar levels as
in control or Gli3–/– limbs.
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