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Fig. 2. Cardiomyocyte transcriptional reprogramming. (A,D) Embryonic expression of
wg (A) and Ih (D) in stage 16 embryos. In situ hybridization
(green), and anti-dMef2 staining (A,D, red) or anti-Tin staining (D, inset,
red). (A) At stage 16, wg is expressed in svp-expressing
cells in segments A5 to A7 (arrowheads)
(Lo et al., 2002). (D) In the
embryonic cardiac tube, Ih is expressed in the two posterior-most
pairs of Tin-myocytes in segment A4, and in the four pairs of Tin-myocytes in
segments A5 and A6 (arrows). Ih is not expressed in
svp-expressing cells (which also do not express Tin, arrowheads), or
in segment A7, or in the anterior and posterior aorta (segments T1 to A3 and
anterior part of segment A4). (B,C) Anti-Wg staining of a 30-hour-APF pupal
cardiac tube. Five pairs of cells express Wg. (C) At higher magnification and
based on their morphology, Wg-expressing cells are identified as the
svp-expressing myocytes of segments A1 to A5 (arrowhead). (E,F)
Ih expression in the adult cardiac tube. Ih is expressed in
all Tin-expressing myocytes of segments A1 to A4 (white arrows), but not in
svp-expressing myocytes (arrowhead). Ih is no longer
detected in segment A5 Tin-expressing myocytes (red arrow). (F) Detail of the
posterior part of an adult cardiac tube. Ih is not expressed in
svp-expressing myocytes (arrowhead), nor in segment A5 Tin-expressing
myocytes (red arrow). (H) Ndae1 expression in the adult heart. Detail
of the posterior part of an adult cardiac tube. Ndae1 is not
expressed in svp-expressing myocytes (arrowhead), nor in segment A5
Tin-myocytes (red arrow), but is expressed in Tin-expressing myocytes of
segments A1 to A4 (only A4 is shown, white arrows). Asterisk in E and H
indicates fat body non-specific staining. (G) Schematic representation of
svp, Ih, Ndae1, Tin and Wg expression in the embryonic and adult
cardiac tubes. (A,D,E,F,H) Confocal images. Scale bars: 50 µm.
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