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Fig. 2. vps33b expression in zebrafish embryos and larvae. (A-I)
Whole-mount RNA in situ hybridization. Sphere stage (A), 10 somite (B) and 24
hpf (C) embryos show diffuse vps33b expression. (D) vps33b
expression at 48 hpf is evident in the developing liver (black arrow) and
proximal intestine (blue arrow). (E) Lateral view of a 72 hpf larva showing
vps33b expression in the liver (black arrow) and proximal intestine
(blue arrow). Weak pancreas expression (white arrow) is also evident. (F,G)
High-power lateral views of 4 dpf larvae processed for vps33b and
ceruloplasmin whole-mount RNA in situ hybridization. Liver (black
arrow) demonstrates a reticular pattern of vps33b expression (black
arrow) (F) compared with a homogeneous pattern of ceruloplasmin
expression (G). Intestinal vps33b expression is also evident (blue
arrow, F). (H) Higher power view of liver depicted in F. (I) Enhanced view of
H outlining putative ducts. (J,K) Histological cross-sections of a 4 dpf larva
processed for vps33b (J) and ceruloplasmin (K) whole-mount
RNA in situ hybridization. These panels show punctate regions of
vps33b expression in presumptive bile ducts (green arrowheads) and a
small number of hepatocytes (red arrowheads). Biliary epithelial cell size and
cytoplasm in these panels are comparable with biliary epithelial cell
ultrastructure (Fig. 5)
(Lorent et al., 2004;
Matthews et al., 2004).
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