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Fig. 6. vps33b knockdown disrupts intestinal vesicle transport. (A-D)
Electron micrographs of enterocytes from 5 dpf wild-type (A) and
vps33b morpholino injected (B-D) larvae. The apical surface is to the
right of each panel. Insets are at twice the magnification of the indicated
regions of cytoplasm. No vesicles can be identified within the wild-type cell
cytoplasm. (C) Multiple vesicles (arrows) are seen in the cytoplasm of
vps33b-deficient enterocyte. Dilated stacks of Golgi cisternae are
also evident within these cells (D). g, golgi; m, mitochondria; n, nucleus.
(E-H) Histological cross-sections from the anterior intestine of 5 dpf
wild-type (E,G) and vps33b morpholino-injected (F,H) larvae that have
ingested the styryl dye AM1-43. Nuclei stained with DAPI. (G,H) Magnified
views from E and F, respectively. There was a 1.8-fold increase in the number
of fluorescent vesicles in the vps33b-deficient larvae (see
Table 4).
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