DEV02141 Supplementary Material

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• Supplemental Figure 1 -

  Fig. S1. Histological analysis of the serosal mesothelium on embryonic and postnatal intestinal tracts. Hematoxylin and Eosin-stained sections from intestinal tracts at E10.5 (A,A¢), E11.5 (B,B¢), E13.5 (C,C¢) and E14.5 (D,D¢), and from adult mouse (F,F¢), and immunohistochemistry for Wt1 at E14.5 (E) and in the adult (G). At E10.5, the mesodermal surface of the gut is missing a simple squamous epithelium, instead cells are irregularly arranged (arrowheads, A,A¢). At E11.5, cells on the mesodermal surface are arranged in a smooth single layer (arrowheads, B,B¢). At E13.5 and later stages, the squamous character of the mesothelium is visible (arrowheads,C-D¢,F,F¢). The presence of mesothelial cells is also demonstrated with an anti-Wt1 antibody (E,G). Scale bars: 25 mm in A-E,G; 10 mm in A¢,B¢-D¢;,50 mm in F; 20 mm in F¢.

• Supplemental Figure 2 -

  Fig. S2. Genetic lineage tracing of Wt1-descendant cells. Immunohistochemistry for b-Gal and desmin, a marker for smooth muscle cells, on sections through the adult gut shows colocalization in the smooth muscle cells of vessels (arrowheads, A-C). Confocal imaging of immunoreacted sections of newborn gut shows co-labeling of b-Gal and SMA (arrowheads, D-F). vism, visceral smooth muscle. Scale bars: in A, 30 mm for A-C.