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Fig. 1. Wt1 and cytokeratins as markers for mesothelial cells in the heart and gut.
(A,B) Expression of Wt1 and cytokeratin mark epicardial mesothelium. (A)
Merged image of Wt1 (green) and cytokeratin (pCK, red) colocalization in
mesothelial cells (arrows) over the heart (h) and the adjacent liver (l) at
E11.5. Boxed area in A is shown in B-B'' to delineate
colocalization. (C-G) Wt1 and cytokeratin also mark serosal mesothelium. (C)
Expression of Wt1 is first observed in the urogenital ridge at E9.5
(arrowheads) but is absent from the mesentery (arrows) and gut tube. (D) At
E10.5, Wt1 staining extends over the mesentery (arrows) but does not cover the
entire gut. (E) Cytokeratin staining is absent from the gut surface, but
residual expression is seen in splanchnic mesoderm of the mesentery (arrows).
Note cytokeratin staining in somatic mesoderm (asterisk) of the body wall.
(F,G) At E11.5, Wt1 (F, arrows) and cytokeratin (G, arrows) staining is
evident on the entire surface of the mesentery and gut tube. Note that only
cytokeratin is expressed in the endodermal linings of the gut (e). lua, left
umbilical artery; m, mesentery; nd, nephric duct; rua, right umbilical artery;
sm, somatic lateral mesoderm. Scale bars: 20 µm in B-B''; and
100 µm for A,C-G.
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