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Fig. 3. Molecular genetic analysis of serosal mesothelial development. (A-D)
Whole-mount analysis of lacZ expression marks the advance of the
serosal mesothelium. (A) At E11.5, lacZ staining is prominent at the
root of mesentery and intestine (small arrows), but is absent from the
herniated gut (large arrows). Note the staining in nephric tubules (black
arrowheads) and the urogenital ridge (white arrowhead). (B) At E12.5,
lacZ-positive cells are seen covering more distal portions of the gut
(arrows). (C) By E14.5, the intestine and mesentery (m) are positive for
lacZ staining. (D) At E18.5, lacZ staining is strong over
the entire gut tube and in the mesentery. (E-E'')
Immunohistochemistry for ß-Gal (E, red) and Wt1 (E', green) reveal
that, at E12.5, most of the serosal mesothelial cells express ß-Gal;
ß-Gal staining colocalizes with Wt1 staining (arrows; E'').
e, endoderm; in, intestine; st, septum transversum. Scale bars: 500 µm in
A-C; 250 µm in D; 50 µm in E-E''.
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