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Fig. 3. Altered cilia morphology on cells of the ventricular system in
Tg737orpk mutant mice. Photomicrographs of brain sections
from wild-type and mutant animals, showing immunolocalization of
acetylated-
-tubulin (green) and polaris (red). White and yellow
arrowheads indicate cilia. (A) Ependymal cilia in wild-type mice are in
well-organized groups, with equal length, whereas cilia on the
Tg737orpk mutant ependyma are fewer in number, shorter and
anisometric. Polaris predominantly localizes to the basal body in the
wild-type ependyma, but is found to accumulate at the cilia tip in the
mutants. (B) Grouped and primary cilia are present on the CP of wild-type mice
and polaris is concentrated at the basal bodies. Polaris accumulates at the
tip of the grouped and primary cilia in Tg737orpk mice.
Cilia often exhibit a large bulb-like structure in which polaris is
concentrated. (C) Scanning electron microscopy of ependymal cilia of normal
and Tg737orpk mutant mice. (D) Cilia on the CP of normal
and Tg737orpk mutants. In mutants, the cilia are
morphologically abnormal with a thickened axoneme. Scale bars: in A, 20 µm;
in B, 10 µm; in C, 15 µm; in D 2.5 µm.
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