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Fig. 4. Defects in cilia beat of the Tg737orpk mutant result in
impaired fluid flow over the ependymal cells. Red and yellow arrowheads label
the ependyma and ependymal apical cilia, respectively. (A,B) DIC (A) and
fluorescence (B) images of wild-type and mutant ependyma. Fluorescence images
were overlaid with the movement of the fluorescently labeled beads, as
recorded by motion tracking (yellow lines, see Movie 1 in the supplementary
material). Movement of the beads propelled by wild-type cilia beating was
rapid and directional, whereas movement of the beads in the mutant samples was
random. Scale bar: 20 µm. (C) Graph showing quantitative analysis of the
flow generated by the cilia in the left (LV) and fourth (4V) ventricles from
mutant and wild-type samples (n=6;
*P<0.005).
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