DEV02150 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. abd-A expression in bicoid and hid bicoid mutants. In situ analysis of abd-A mRNA distribution in wild-type (A), bicoid (B) and hid bicoid mutants (C). In wild-type embryos, abd-A expression is restricted to the anterior abdomen of wild-type embryos (A) (parasegments 7-12) (Karch et al., 1990; Macias et al., 1990). In hid bicoid double mutants, expression of abd-A is extended towards the anterior (C), providing further evidence for mis-specification. In bicoid single mutants, almost the entire extension of abd-A-expressing and mis-specified tissue is subject to tissue clearance by cell death (B, see arrow). Thus, this analysis provides further evidence that mis-specification of cells causes cell death in bicoid mutants.

• Supplemental Figure 2 -

  Fig. S2. Mis-specification in oskar embryos. Wild-type (A), oskar (B,C) and hid oskar (D) embryos were labeled with anti-Antennapedia (Antp) antibodies (Condie et al., 1990) as marker of mis-specification. Compared with stage 5 wild-type embryos (bracket in A), the distribution of Antp is dramatically expanded and shifted towards the posterior in oskar embryos (bracket in B). Thus, mis-specification, as visualized by Antp labelling, includes the entire posterior half of oskar embryos, and overlaps with hid expression (Fig. 3C) and TUNEL labeling (Fig. 1I). In stage 15 oskar embryos, tissue clearance indicative of cell death affects most of the Antp-expressing tissue (C, arrows). By contrast, this clearance is strongly reduced in hid oskar double mutants (D). Thus, the mis-specified abdominal tissue expressing Antp survives in the double mutant. In summary, similar to bicoid mutants, mis-specified cells induce hid to undergo cell death in oskar mutants.