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Fig. 4. Tc'Krjaws is an amorphic mutation, and the homeotic transformations in jaws are epistatic over those in Tc'gt RNAi embryos. (A) Tc'Krjaws mutant embryo in which the defective Tc'Kr mRNA additionally has been depleted by RNAi. The phenotype of this embryo is not stronger than in Tc'Krjaws alone, indicating that the Tc'Krjaws mutant itself fully inactivates the gene. (B) Tc'Krjaws mutant embryo in which the Tc'gt gene has been inactivated by RNAi. The normally present maxilla and labium are fully developed, followed by one ectopic maxillary and one labial segment. Thus, the homeotic effect of Tc'Kr inactivation is epistatic over that observed in Tc'gt RNAi larvae. Because Tc'gt also results in segmentation defects, the 2nd ectopic pair of maxillary and labial segments are incomplete or missing. Arrows indicate maxillary structures; arrowheads labial appendages; pp, pygopode. (C) Phenotype of a Tc'gt RNAi larva. In addition to abdominal segmentation defects, maxillary and labial segments are transformed into thoracic segments.





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