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Fig. 6. The first five Tc'eve stripes are formed normally in Tc'Krjaws. Ventral views of stage-matched wild-type (A-D) and Tc'Krjaws (E-H) embryos. The youngest wild-type (A,B) and all Tc'Krjaws (E-H) embryos have been triply labelled for Tc'eve (brown), Tc'en and Tc'gt mRNA (both blue); in the older wild-type embryos (C,D), only Tc'eve (brown) and Tc'en or Tc'wg (blue) are labelled. Tc'gt expression was used to identify mutant embryos: the posterior giant domain(s) present in wild-type germbands (A,B) are missing in Tc'Krjaws (E,F); black arrowheads indicate the position of these domains. In older Tc'Krjaws embryos, two ectopic Tc'gt domains appear in the first and third thoracic segments (grey arrowheads in F-H). In Tc'Krjaws mutant germbands (E,F), the primary eve stripes 3 and 4, and the secondary segmental stripes of eve2 form in a similar way to wild type (A,B) (stripes eve1a and eve1b are partially obscured by en stripes and the maxillary Tc'gt expression). Later on, eve3 splits into segmental stripes, while eve5 becomes detectable (C,G). The segmental stripes 4a and 4b remain imperfect in Tc'Krjaws, while eve5 forms a sharp anterior boundary (H).





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