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Fig. 6. pelo mutant ovaries exhibit age-dependent egg chamber degeneration. (A) Part of a 2-day-old pelo¹ mutant ovariole labeled for Hts (red, follicle cells) and DAPI (nuclei, blue), showing normal nurse cell morphology and the oocyte (arrowhead). (B) Part of a 7-day-old pelo¹ mutant ovariole labeled for HtsRC (green, ring canals) and DAPI (nuclei, blue), showing condensed nuclei of degenerating nurse cells in the egg chamber, in which the oocyte is indicated by an arrowhead. (C) Part of a 7-day-old pelo¹ mutant ovariole labeled for Hts (red, follicle cells) and DAPI (nuclei, blue), showing several degenerating and growth-arrested egg chambers (arrowheads). (D) Part of an ovariole labeled for lacZ (green) and DAPI (nuclei, blue), showing a degenerating (unusually condensed nurse cell nuclei) and growth-arrested pelo¹ mutant germline cyst (arrowhead, no lacZ expression). Scale bars: 10 µm.

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