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Fig. 6. Localization of amino acid substitution mutants of CPC in roots, and
phenotypes of the mutant plants. (A,D,G,J,M) CLSM images of GFP fluorescence
(green) and PI fluorescence (red) in the root epidermis of 5-day-old
seedlings. (B,E,H,K,N) Root hair phenotypes of 5-day-old seedlings.
(C,F,I,L,O) Trichome phenotypes of 9-day-old seedlings. K6A:K9A, L77V and K79A
moved from hairless cells to hair cells, and accumulated in the nuclei of both
cell types (A,D,G). The plants developed ectopic root hairs (B,E,H) but no
trichomes, thus resembling lines expressing CPC:GFP (C,F,I). The capability
for cell-to-cell movement was reduced in M78A, but it accumulated in the
nuclei (J). M78A formed ectopic root hairs but no trichomes (K,L). The W76A
form of the protein did not move from hairless cells to root hair cells, and
GFP-derived fluorescence was dispersed throughout the cells (M). Normal root
hair formation and trichome formation were observed in this mutant (N,O).
(P,Q) Alignment of the N termini, and of the C-terminal regions of the Myb
domains, respectively, of CPC and homologous small Myb proteins. Small Myb
protein sequences were compiled from Schellmann et al.
(Schellmann et al., 2002)
(TRY) and Kirik et al. (Kirik et al.,
2004a; Kirik et al.,
2004b) (ETC1 and ETC2). The S1 region is unique to CPC, whereas
W76 and M78 in S2 are conserved in all small Myb proteins. Scale bars: 50
µm in A; 200 µm in B; 1 mm in C.
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