spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Fgfr1-IIIc and Fgfr2-IIIb isoforms are expressed by migrating PGCs. (A) cDNA prepared from FACS-sorted E10.5 PGCs and somatic cells was subjected to PT-PCR. The purity of the preparations was confirmed by expression of the PGC marker gene stella, and the somatic marker genes steel and Twist1. Lane 1, E10.5 PGC cDNA (PGC); Lane 2, E10.5 somatic cell cDNA (SC); Lane 3, E10.5 whole embryo cDNA (WE); Lane 4, RT(-) E10.5 whole embryo cDNA [WE(RT-)]. (B) The expression patterns of all FGFR isoforms were assayed by RT-PCR. Fgfr1-IIIc and Fgfr2-IIIb were found to be expressed by E10.5 PGCs (lane 1; lanes as in A). PGC-derived PCR products were sequenced and verified that these bands truly represented the expression of each FGFR. The illustration shows the potential FGF ligands that could activate each FGFR.





Right arrow Return to article